Protocol for modeling acquired resistance to targeted therapeutics in adherent and suspension cancer cell lines via in situ resistance assay

2024-10-5

Protocol for quantification of intercellular connection transmission in ebolavirus infections using ImageJ

2024-10-5

Protocol for live neuron imaging analysis of basal surface fraction and dynamic availability of the dopamine transporter using DAT-pHluorin

2024-10-4

Protocol for isolating CD163+ Kupffer cells from human liver resections

2024-10-4

Protocol for the simultaneous isolation of DNA, RNA, and miRNA from a single archived Kaposi sarcoma biopsy

2024-10-4

Protocol for analyzing protein-protein interactions by split-luciferase complementation assays in human cell lysates

2024-10-4

Protocol for analyzing plasma cell-free DNA fragment end motifs from ultra-low-pass whole-genome sequencing

2024-10-3

Protocol for simultaneous detection of mRNAs and (phospho-)proteins with ARTseq-FISH in mouse embryonic stem cells

2024-10-1

A protocol for time-resolved transcriptomics through metabolic labeling and combinatorial indexing

2024-10-1

Protocol for culturing patient-derived organoids of cervical cancer

2024-10-1

Protocol for detecting endogenous GPCR activity in primary cell cultures using ONE-GO biosensors

2024-10-1

Protocol to process follow-up electronic medical records of peritoneal dialysis patients to train AI models

2024-10-1

Protocol to calculate the synergy of drug combinations in organoids and primary cells from murine tumors

2024-10-1

Protocol for analyzing functional gene module perturbation during the progression of diseases using a single-cell Bayesian biclustering framework

2024-9-30

Protocol for an open-source system to integrate calcium imaging, pupillometry, and locomotion-estimated tracking in head-fixed mice

2024-9-30

Protocol for generating human CAR-engineered macrophages by Vpx-containing lentivirus

2024-9-28

Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from off-the-shelf antibodies

2024-9-28

Protocol for the acquisition and maturation of oligodendrocytes from neonatal rodent brains

2024-9-28

Protocol for the efficient and inducible generation of CRISPR-Cas9-edited human cortical neurons from the iCas9-iNgn2 hPSCs

2024-9-28

Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer

2024-9-28

Protocol for the processing, cryopreservation, and biobanking of patient-derived cells and tissues

2024-9-27

Protocol for generating a 3D culture of epiblast stem cells

2024-9-27

Protocol for hydroxylating aryl chlorides catalyzed by photouranium with water under ambient conditions

2024-9-27

Protocol to study oxygen dynamics in the in vivo mouse brain using bioluminescence microscopy

2024-9-26

Protocol for high-resolution 3D visualization of insect regenerating legs through micro-computed tomography

2024-9-26

Protocol for a non-surgical model of perineural invasion for assessing neural drivers of cancer aggressiveness in mice

2024-9-26

Protocol for the neonatal intracerebroventricular delivery of adeno-associated viral vectors for brain restoration of MECP2 for Rett syndrome

2024-9-25

Protocol to study cis-regulatory activity of GWAS loci for specific gene promoters in human primary astrocytes using luciferase reporter assay

2024-9-25

Protocol to establish mouse esophageal epithelial whole mounts for lineage tracing or immunofluorescent analysis

2024-9-25

Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display

2024-9-24

Protocol for developing an explosion-propeller hybrid driving underwater robot for AI-based concrete overhaul in real marine environments

2024-9-23

Protocol to detect spatio-molecular profiles underlying neuroimaging features in the human cerebellum

2024-9-23

Protocol for analysis of plasma proteomes from patients with hepatocellular carcinoma receiving combination therapy

2024-9-23

Protocol to quantify the activation dynamics of tumor-associated T cells in mice by functional intravital microscopy

2024-9-21

Protocol to establish patient-derived xenograft and organoid for radiosensitivity of esophageal squamous cell carcinoma

2024-9-21

Protocol for assessing murine cell doublet engagement and subsequent effects using flow cytometry and imaging flow cytometry

2024-9-21

Protocol for preparing metabolically reprogrammed human CAR T cells and evaluating their in vitro effects

2024-9-21

Protocol for formation, staining, and imaging of 3D breast cancer models using MicroTissues mold systems

2024-9-20

Protocol to monitor live-cell, real-time, mitochondrial respiration in mouse muscle cells using the Resipher platform

2024-9-20

Protocol for rapid evaluation of antibody-mediated protection in macrophages using immunofluorescence techniques

2024-9-19

Protocol for extracting oil from Ghana shea nuts using solvent extraction

2024-9-19

Protocol for the longitudinal study of neuroinflammation and reactive astrocytes in Lcn2CreERT2 mice

2024-9-19

Click-chemistry-based protocol for detecting 4-octyl-itaconate-alkylated proteins in primary mouse macrophages

2024-9-19

Protocol for detecting glycoRNAs using metabolic labeling and northwestern blot

2024-9-19

Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin

2024-9-19

Protocol for monitoring phagocytosis of cancer cells by TAM-like macrophages using imaging cytometry

2024-9-19

Protocol for quantum dot-based cell counting and immunostaining of pulmonary arterial cells from patients with pulmonary arterial hypertension

2024-9-18

Protocol to process fresh human cerebral cortex biopsies for patch-clamp recording and immunostaining

2024-9-17

Protocol to infer and analyze miRNA sponge modules in heterogeneous data using miRSM 2.0

2024-9-17

Protocol for the generation, characterization, and functional assays of organoid cultures from normal and cancer-prone human esophageal tissues

2024-9-14